A molecular basis underpinning TRBV28 T-cell receptor recognition of MR1–antigen

W Awad; NA Gherardin; L Ciacchi; AN Keller; L Liu; DP Fairlie; J McCluskey; DI Godfrey; J Rossjohn

Journal article

A molecular basis underpinning TRBV28 T-cell receptor recognition of MR1–antigen

W Awad, NA Gherardin, L Ciacchi, AN Keller, L Liu, DP Fairlie, J McCluskey, DI Godfrey, J Rossjohn

Journal of Biological Chemistry | Elsevier | Published : 2025

DOI: 10.1016/j.jbc.2025.110416

Download PDF

Abstract

Mucosal-associated invariant T (MAIT) cells express a TRAV1-2+ T-cell receptor (TCR) that recognizes microbial vitamin B2 derivatives presented by the major histocompatibility complex class I–related molecule (MR1). Most MAIT TCRs incorporate a biased TCR-β repertoire, predominantly TRBV20-1 and TRBV6, but some utilize other trbv genes, including TRBV28. A second conserved, albeit less frequent TRAV36+ TRBV28+ T-cell population exhibits MAIT-like phenotypic features but use a markedly distinct mode of MR1–antigen (Ag) recognition compared with MAIT TCR–MR1 binding. Nevertheless, our understanding of how differing TCR gene usage results in altered MR1 binding modes remains incomplete. Here, b..

View full abstract